United in research, we drive success
in the fight against cancer.
Twinning for excellence to advance research in the activation
of anti-tumor immune response after electrochemotherapy
combined with gene electrotransfer of pDNA encoding ICIs
Project objectives
- To develop and implement the state-of-the-art, high-throughput, multi-omics in vitro and in vivo research platform
- To delineate the molecular and cellular mechanisms at the in vitro and in vivo level governing the type of cell death after electrochemotherapy.
- To translate the local efficacy of electrochemotherapy combined with gene electrotransfer of pDNA encoding ICIs into a systemic one.
- To raise the research capacity and excellence
- To increase the administrative and research management skills
- To build and maintain a sustainable communication and dissemination channels for the project results
The ZAP Cancer Project is pioneering advanced cancer therapies by combining electrochemotherapy with gene electrotransfer techniques. This innovative approach aims to enhance immune system responses to eliminate tumors more effectively.
Using state-of-the-art techniques like high-throughput multi-omics platforms, CRISPR/Cas9 gene targeting, and RNA sequencing, researchers aim to uncover the intricate mechanisms of cell death and anti-tumor immune responses.
A key focus of the project is inducing immunogenic cell death, a process where cancer cell destruction triggers an immune response. By leveraging techniques like electrochemotherapy with agents such as bleomycin, cisplatin and oxaliplatin, the project aims to enhance the visibility of cancer cells to the immune system, fostering a robust anti-tumor response.
The project highlights the use of gene electrotransfer to locally, in the tumor, produce monoclonal antibodies targeting immune checkpoints like CTLA-4 and PD-1, which are critical in reactivating the immune system to recognize and destroy cancer cells. By combining this approach with electrochemotherapy, the project seeks to overcome resistance and maximize treatment effectiveness.
With a network of leading institutions across Europe, the project is fostering knowledge exchange and leveraging advanced imaging, molecular biology, and bioinformatics to push the boundaries of cancer research.
The project aims to empower students and early-career scientists by providing them with the tools, resources, and mentorship needed to become leaders in cancer research and treatment development.
Latest News
ZAP Cancer Project Member Showcases Innovative Research at Knowledge for Growth Conference in Antwerp
On 8 May 2025, Nick Geukens from KU Leuven delivered a scientific presentation at the Knowledge for Growth life sciences conference held at the Flanders Meeting & Convention Center in Antwerp, Belgium. The event, one of Europe’s leading gatherings for biotech, pharma, medtech, and health innovation professionals, attracted researchers, industry leaders, and investors from across the continent. The ZAP Cancer Project, coordinated by the Institute of Oncology Ljubljana and funded under the Horizon Europe programme, aims to advance cancer therapy by combining electrochemotherapy with gene electrotransfer of plasmid DNA encoding immune checkpoint inhibitors to enhance systemic anti-tumor immune responses. At the conference, the project’s speaker shared recent progress and insights into the molecular and translational aspects of this innovative approach, highlighting opportunities for collaboration and future clinical impact within the European life sciences ecosystem.